Sunday, 31 July 2016

MFC, house Layer, third weekend 31.7.2016


At the end of the third weekend, we (Sara, Benjamin, Z) compared results and the best option in the test of cultures is second or B option of cultures YC-X16


Cultures are mainly Lactobacillus bulgaricus and Streptococcus thermophilus

 

For more precise characteristics like temperature range of culture YC-X16, a technical sheet from the producer CHR Hansen is copied on the post.

A series of graphs is pasted below: y-axis representing current in mV, x-axis are days. As graphs might contain experimental error and measurements in days is made per day and not recalculated by hours (measurements were made mostly in the afternoon or evening from 19 to 21 h) they determine that further experiments will be made with cultures YC-X16 (B)

- cultures  ABY-3 (A) + YC-X16 (B)



cultures ABY-3 (A)
cultures YC-X16 (B) 
different jars



Small deviations are shown, which are minor - more all less measurement we can look as a continuous line.
Results are better with small percentage of culture YC-X16 (B). Additional tests with bigger quantity and even smaller would be nice


Next tests:

- different volumes
- different sizes of electrodes (in diameter) and also in future different materials (test ob bio-charcoal etc.)
- negative tests (meat without cultures YC-X16 and just cultures YC-X16)
- same combinations 3 x test

Tuesday, 26 July 2016

MFC, house Layer, second weekend 26.7.2016

All the measurement are slowly rising or descending. With resistor also current was measured.




And some photos:







Sunday, 17 July 2016

MFC - first weekend


First tests of bioreactor powered on horse meat. With the courtesy of  House Layer in Kranj, we started using a nice place for one-month testing. Before coming to Kranj preparations were mostly getting all materials and equipment needed for tests.  Methylan blue, different measuring pots (Mikro+Polo), Lactobacillus culture (Biotechnical centre Naklo), Agar, agar (food store), Inox rods for electrodes (MDM), carbon (Samson), digital multimeter (IC Elektronik), different plastic containers ... (hardware shop).







Salt bridge initial formula 300 ml of water, 30 ml of salt (NaCl) and 5 g of Agar agar. Later ratio was 10% of salt and 5% of agar agar.





Minced meat was processed in a blender to smaller pieces. Meat 17% (1/2 kg and 2 1/2 of water)

Lactobacillus - bulgaricus and Streptococcus thermophilus



Toner was added to epoxy glue




Preparation of bioreactor was mostly handled by Sara Zupanc and Benjamin Drakslar who were also suggested making additional tests on the different percentage of content of the cultures.






















And the results after the end of the weekend.





Saturday, 9 July 2016

micro fuel cell - preparation

We had a wonderful meeting with Sara and Benjamin in the third biggest brewery in Slovenia - Kranjska pivnica. A short list of ingredients and possible links is added below. Tests will start soon.




mediators:

microbiological cultures
Lactobacillus: 
biotehnični center naklo http://www.bc-naklo.si/index.php?id=215

cheesemaking

agar agar